Specific Determination of β-Galactocerebrosidase Activity via Competitive Inhibition of β-Galactosidase
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چکیده
منابع مشابه
Specific Determination of -Galactocerebrosidase Activity via Competitive Inhibition of -Galactosidase
BACKGROUND: The determination of cellular -galactocerebrosidase activity is an established procedure to diagnose Krabbe disease and monitor the efficacy of gene/stem cell– based therapeutic approaches aimed at restoring defective enzymatic activity in patients or disease models. Current biochemical assays for -galactocerebrosidase show high specificity but generally require large protein amount...
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Introduction Transient expression of cloned DNA sequences into mammalian cells is a commonly used tool in molecular biology for the investigation of transcriptional activity of eukaryotic promoters. However, in most cases the natural product of the promoter cannot be assayed in a quantitative fashion. In these instances, the promoter is joined to a reporter gene, which encodes for a protein wit...
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es, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequencebased families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2009
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2008.115873